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Neurohistology Domain

Project Summary

Goals

This core uses a series of relatively high throughput procedures to establish the functional anatomy of the normal and mutant brain.

Approach

A series of relatively simple stains is conducted on a representative brain of each pedigree of test class mice. These stains will assess the cytoarchitectonics, myelinated fiber pathways, terminal fields, astroglia and neuronal populations, activity state, and proliferative populations and neuropathology in brain.

The stains we will employ are: cresyl violet, osmium tetroxide, acetylcholinesterase, anti-glia fibrillary acidic protein and anti-NeuN immunocytochemistry, cytochrome oxidase histochemistry, cell birthdating with BrdU immunohistochemistry, and neuropathology using anti-ubiquitin immunohistochemistry. In addition we are exploring the use of other stains in our phenotyping protocol that might serve as markers of aging (such as PAS, and immunocytochemistry using anti-laminin, and anti-chondroitin sulfate proteoglycan antibodies, and stains to label dying cells).