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Projects: Neuromutagenesis
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Home | News | Navigate Site | About TMGC | Contact Us Projects: Neuromutagenesis |
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Approach
The TMGC is currently screening for recessive mutations affecting neurobiology, behavior, and aging phenotypes in several segments of the genome. Two of the screens (40 cM of mid-Chr 10 and the distal of Chr 15) use dominant and recessively marked inversions to make ENU-mutagenized chromosomes homozygous in the great-grandprogeny of ENU-treated males. A screen of a 10-15 cM segment of the proximal X-Chromosome is being done using a hemizygosity strategy, and a screen of all of Chr 19 is being carried out by molecular genotyping of ENU-treated consomic mice.
The design of each autosomal screen is such that a visibly marked "test class" of mice, who are homozygous for a mutagenized chromosome in the region of interest, is produced in the G3 generation. Within each pedigree, such G3 animals are then crossed to generate a population of G4 animals, subsets of which can be aged or sent through phenotyping assays. Thus, the larger G4 (or G5) test-class populations provide animals with which to screen for more statistically sensitive or subtle mutations to add to the collection of visible phenotypes and lethal phenotypes easily recognized in the crosses.
TMGC Mutagenesis Site Map
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Page last updated: Monday, November 17, 2003.