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Goals
The aim of this screen is to identify mutations that alter susceptibility to seizures.
Approach
Two types of screens are being employed:
- Seizure induction by hyperthermia:
In humans, febrile seizures occur in infants (6 months to 5 years) and are associated with a rise in body temperature. We will simulate febrile seizures in infant mice (14-15 days old) by subjecting them to whole body hyperthermia.
- Seizure induction by methyl-beta-carboline 3-carboxylate:
Methyl-beta-carboline 3-carboxylate (&beta-CCM) is an inverse agonist of the GABA-A receptor. Mice experience seizures of varying severity after receiving peritoneal injections of &beta- CCM.
Protocols and procedures